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KMID : 0380020040190030169
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 3 p.169 ~ p.173
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Separation and Performance Test of Whitening Agent in Rhodiola Sachalinensis
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Choi Doo-Young
Ahn So-Young
Lee Seung-Ki
Han Jung-Sun
Kim Eun-Cheol
Lee Hyang-Bok
Shin Jeong-Hyun
Kim Eun-Ki
Row Kyung-Ho
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Abstract
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The Rhodiola Sachalinensis 5 g were mixed and extracted with methanol 150 at the room temperature for 12 h. The effluents were collected and grouped into the two. Un this experimental condition, the mobile phase composition were linearly changed as follows; water/methanol : 90/10 - 30/70 (vol. %, for 5 min), 30/70 - 10/90 (vol. %, for 15 min) and an analytical column (3.9 25 em, 15 particle size, and 300 pore size) was utilized. The performance of the extracted Rhodiola Sachalinensis as a whitening agent was not favorable, so it classifies the Rhodiola Sachalinensis extractions with two fractions and collects each fraction for whitening agent assay. For the in-vivo melanin production ratio assay that used melanin-a cell in 10 ppm concentration, it was 58.6%, the first fraction of the effluents collected between 1.0 and 4.0 min, while it was 60％ between 10.4 and 17.6 min for the second fraction, which were more efficient than that of arbutin, 45.6%.
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KEYWORD
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Rhodiola sachalinensis, HPLC, melan-a, melanin production ratio, tyrosinase inhibitory activity
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